C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/134
C12P 13/06 (2006.01) C12P 1/00 (2006.01) C12P 13/04 (2006.01) C12P 19/36 (2006.01)
Patent
CA 1139697
ABSTRACT OF THE DISCLOSURE Water-soluble .alpha.-ketocarboxylic acids are continuously converted into the corresponding amino acids in a diaphragm reactor. The conversion is carried out in the presence of a substrate-specific dehydrogenase, ammonium ions and, as the coenzyme, nicotinamide-adenine dinucleotide (NAD+/NADH), which had been increased in the molecular weight by linkage to a water- soluble polymer. At the same time NADH is continuously regener- ated from NAD+ in the presence of a formate dehydrogenase and formate ions. The diaphragm must have an average pore diameter of 1 to 3 nm. From 0.1 to 10 mmoles/litre of NAD+/NADH linked to a poly-oxyethylene having an average molecular weight between 500 and 50,000 are used as the coenzyme. A substrate flow containing 50 to 100% of the maximally soluble quantity (but not more than 200 mmoles per litre) of the .alpha.-ketocarboxylic acid to be reacted, in the form of a water-soluble salt, a quantity of ammonium ions approximately equimolar to the quantity of substrate and 100 to 6,000 mmoles/litre of a formate is continuously fed to the reactor. A differential pressure of 0.1 to 15 bars is maintained across the diaphragm. A filtrate flow containing the amino acid formed is continuously drawn off behind the diaphragm.
356910
Buckmann Andreas
Kula Maria-Regina
Leuchtenberger Wolfgang
Wandrey Christian
Wichmann Rolf
Aktiengesellschaft Degussa
Gesellschaft Fur Biotechnologische Forschung Mbh (gbf)
Marks & Clerk
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