C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/64 (2006.01)
Patent
CA 2545416
The invention relates to an optimized method for the production of PUFAs by cultivating microorganisms of the order thraustochytriales in a fermentation medium that is pH-stabilized using calcium carbonate and comprises 3 to 15 g/L of CaCO3, whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention particularly relates to novel optimized media having a different CaCO3 content. By using adequate quantities of CaCO3, the process can be significantly simplified during fermentation while greater quantities of DHA can be obtained at an increased oil content in the biomass, allowing thraustochytriales microorganisms to be fermented without controlling the pH, thereby substantially improving and significantly simplifying PUFA production.
L'invention concerne un procédé optimisé pour réaliser des acides gras polyinsaturés (PUFA) par culture de micro-organismes de l'ordre des Thraustochytriales dans un milieu de fermentation à pH stabilisé par du carbonate de calcium, avec 3-15 g/L de CaCO3 et par isolation subséquente des PUFA des micro-organismes et/ou du milieu. L'invention concerne en particulier de nouveaux milieux optimisés à teneur en CaCO3 variée. L'utilisation de quantités de CaCO3 correspondantes permet une simplification notable du procédé pendant la fermentation. On obtient en outre une augmentation des quantités de DHA pour une teneur en huile accrue dans la biomasse. La fermentation de micro-organismes des Thraustochytriales est ainsi possible sans régulation du pH, d'où une réalisation de PUFA sensiblement améliorée et simplifiée.
Luy Markus
Rusing Matthias
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Nutrinova Nutrition Specialties & Food Ingredients Gmbh
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