C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/128, 167/45
C12N 9/64 (2006.01) C12Q 1/37 (2006.01)
Patent
CA 1197778
ABSTRACT The present invention provides a process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy, based on the cellular principle, wherein leukocytes of a sample to he investigated are incubated with an allergen or another stimulation factor of degranulation, such as anti-IgE, in an aqueous medium, the leukocytes are then separated off and the remaining solution is incubated with a compound of the general formula:- Y-X-C in which Y is an amino acid or a peptide containing 2 or 3 amino acids, the amino acids optionally carry- ing a conventional protective group on the amino end, X is arginine or lysine and C is a chromogenic residue, in the presence of a buffer effective at pH 6.3 to 8.0 and the liberation of the chromogenic residue is measured.
424570
Stahl Peter
Wilhelms Otto-Henning
Wunderwald Peter
Boehringer Mannheim G.m.b.h.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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