Process for the determination of antibodies

G - Physics – 01 – N

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167/44

G01N 33/537 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1335786

For the determination of antibodies based on an immunoassay technique by incubation with at least three receptors R1, R2 and R3 which are present dissolved in a liquid phase and of which R1 is an antigen which is capable of being specifically bound to the antibody to be determined, R2 mediates the binding to the solid phase and R3 carries a label, separation of the complex which forms from the solution by binding to a solid phase and measurement of the label in one of the phases, a conjugate is used as the receptor R2 composed of a receptor capable of specific binding to R1 and a substance S1, which can be specifically bound, and a conjugate of a receptor which can specifically bind to R1 and a label is used as R3, wherein the immobilization of the complex which forms is mediated by binding to a component of the solid phase which can specifically bind to S1.

604940

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