Process for the enzymatic determination of free and...

C - Chemistry – Metallurgy – 12 – Q

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150/3.3

C12Q 1/60 (2006.01) G01N 33/92 (2006.01)

Patent

CA 1174149

ABSTRACT OF THE DISCLOSURE Cholesterol is determined by incubating a sample suspec- tod of containing cholesterol in an aqueous medium with cholester- ol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising choloaterol oxidase and a system for the determination of H2O2 or for the determination of cholestenone are provided.

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