Process for the enzymatic treatment of substrates

Details Process for the enzymatic treatment of substrates Process for the enzymatic treatment of substrates

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/16 (2006.01) G01N 33/535 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2047235

- 1 - A b s t r a c t For the enzymatic treatment of substrates, the substrate to be treated is brought into contact with a biocatalyser which was obtained by combining, in order to produce a fusion protein, a gene which codes for a biologically active substance and a DNA fragment which codes for a binding peptide which can interact with a carrier material then inserting the combined gene and DNA fragment into a suitable vector, transforming it in a suitable organism, culturing the organism, lysing the cells and bringing the lysate into contact with a carrier material which is capable of binding the binding peptide whereby the fusion protein binds to the carrier material by intermolecular interactions and the enzymatically treated substrate is subsequently isolated.

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