Process for the in vitro replication of hcv

Details Process for the in vitro replication of hcv Process for the in vitro replication of hcv

C - Chemistry, Metallurgy

12

N

C12N 7/00 (2006.01) C12N 5/071 (2010.01) A61K 39/29 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2334767

The invention relates to a use of a culture medium containing: one or several mammalian plasma or sera; a chemical or biological compound having an antioxydative property and/or differentiating property, such as DMSO, retinoic acid, vitamin, for example vitamin E, or selenium; and/or one or several corticoids for the in vitro hepatitis C virus replication in primary mammalian hepatocytes.

L'invention concerne l'utilisation d'un milieu de culture contenant au moins un plasma ou sérum mammifère, un composé chimique ou biologique possédant une propriété antioxydante et/ou une propriété de différenciation, tel que le diméthylsulfoxyde (DMSO), l'acide rétinoïque, une vitamine, par exemple la vitamine E, ou du sélénium, et/ou au moins un corticoïde, dans la réplication in vitro du virus de l'hépatite C dans des hépatocytes primaires de mammifères.

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