Process for the isolation and characterisation of a gene...

C - Chemistry – Metallurgy – 12 – N

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195/39, 195/1.12

C12N 9/80 (2006.01) C12N 1/20 (2006.01) C12N 15/55 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1338440

The present invention relates to a process for the isolation and characterisation of a gene enzyme system for the inactivation of the herbicide phenmedipham. The enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying of the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.

600153

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