Process for the isolation of glycosylated interleukine-2

Details Process for the isolation of glycosylated interleukine-2 Process for the isolation of glycosylated interleukine-2

C - Chemistry, Metallurgy

07

K

530/15.14, 530/3

C07K 14/55 (2006.01) C07K 1/22 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2001107

ABREGE-DESCRIPTIF L'invention concerne un procédé d'isolement d'interleukine-2 glycosylée à partir d'une fraction riche en interleukine 2, issue du surnageant de culture de cellule CHO recombinées. Ce procédé comprend les étapes successives suivantes : a) séparation d'une fraction riche en interleukine-2 du surnageant de culture; b) chromatographie d'échange cationique; c) chromatographie d'interaction hydrophobe; d) chromatographie d'exclusion. L'invention a également pour objet l'interleukine-2 glycosylée susceptible d'etre obtenue par ce procédé. Pas de figure.

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