Process for the isolation of polyhydroxyalkanoates (phas)...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/62 (2006.01)

Patent

CA 2599990

A process for extracting and recovering polyhydroxyalkanoates (PHAs) from cellular biomass comprising the steps of: extracting the polyhydroxyalkanoate with at least one non-halogenated solvent which is non-aggressive to the environment; heating the cellular biomass, in order to form a suspension comprising PHA solvent with the dissolved PHA and insoluble residues; recovering the solvent enriched with PHA; injecting the PHA-enriched solvent in a vapor flow in order to rapidly promote the complete precipitation of PHA in water, while processing the solvent evaporation; depleting the residual solvent; separating the purified PHA particles from the suspension and drying them.

L'invention concerne un procédé d'extraction et de récupération de polyhydroxyalkanoates (PHA) présents dans la biomasse cellulaire, englobant les opérations suivantes: extraction du polyhydroxyalkanoate au moyen d'au moins un solvant non halogéné, non agressif pour l'environnement; chauffage de la biomasse cellulaire dans le but de former une suspension comprenant le solvant PHA avec le PHA dissous et les résidus insolubles; récupération du solvant enrichi en PHA; injection du solvant enrichi en PHA dans un flux de vapeur afin de favoriser la précipitation complète du PHA dans l'eau tout en traitant le solvant évaporé; épuisement du solvant résiduel; séparation des particules purifiées d PHA de la suspension et séchage desdites particules.

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