Process for the manufacture of oligonucleotides

Details Process for the manufacture of oligonucleotides Process for the manufacture of oligonucleotides

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01)

Patent

CA 2721969

A process for manufacturing an oligonucleotide which comprises removing .beta.3-eliminating phosphorus-protecting groups, in particular .beta.-cyanoethyl protective groups from a protected oligonucleotide, wherein said removing comprises contacting the protected oligonucleotide with an amine solution in a solvent which preferably does not consist of pyridine, wherein the conjugate acid of the amine has preferably a pKa of greater than 11.5, and wherein the concentration of the amine in the solution is less than 0.5 mole/liters.

La présente invention concerne un procédé pour fabriquer un oligonucléotide qui comprend lélimination de groupes protecteurs de phosphore b, en particulier des groupes protecteurs ß-cyanoéthyle dun oligonucléotide protégé, ladite élimination comprenant la mise en contact de loligonucléotide protégé avec une solution damine dans un solvant qui nest de préférence pas constitué de pyridine, lacide conjugué de lamine ayant de préférence un pKa de plus de 11,5, et la concentration de lamine dans la solution étant inférieure à 0,5 mole/litre.

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