Process for the microbial conversion of phytosterols to...

C - Chemistry – Metallurgy – 12 – P

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C12P 33/00 (2006.01)

Patent

CA 2326121

A novel process for the fermentation of phytosterol compositions to androstenedione (androst-4-ene-3, 17-dione, AD) and/or androstadienedione (androsta-1, 4-diene-3, 17-dione, ADD) is disclosed. The process utilizes the micro-organism Mycobacterium MB 3683, and selected suitable solubilizing agents such as polypropylene glycol or silicone for solubilizing the phytosterol compositions at high concentrations in the nutrient medium. The innoculum of Mycobacterium MB 3683 is grown in a nutrient medium comprising Refiners molasses and inorganic salts.

Nouveau procédé de fermentation de compositions de phytostérol en androstènedione (androst-4-ène-3,17-dione, AD) et/ou en androstadiènedione (androsta-1,4-diène-3,17-dione, ADD). Ledit procédé repose sur l'utilisation du micro-organisme Mycobacterium MB 3683 et sur des agents solubilisants appropriés sélectionnés tels que le propylène glycol ou le silicone pour solubiliser les compositions de phytostérol à des concentrations élevées dans le milieu nutritif. L'inoculum de Mycobacterium MB 3683 est cultivé dans un milieu nutritif comportant de la mélasse de raffinerie et des sels inorganiques.

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