Process for the physical depolymerization of...

C - Chemistry – Metallurgy – 08 – B

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C08B 37/08 (2006.01) C08J 11/22 (2006.01)

Patent

CA 2446695

The invention relates to a process for the depolymerization of glycosaminoglycanes characterized by the use of high-energy radiation in the presence of an organic compound selected from the group consisting of ethers, alcohols, aldehydes, amides and formic acid. The invention also relates to intermediate depolymerized heparin having Mw comprised between 1,000 and 5,500, absorbance at 400 nm below 0.300 and ratio S03-/COO- equal to or higher than in the starting heparin. The intermediate depolymerized heparin can be dissolved in a buffer solution and fractionated by Gel Permeation for obtaining the desired Molecular Weight.

L'invention porte sur un procédé de dépolymérisation de glycosaminoglycanes caractérisé par l'utilisation de rayonnement à haute énergie en présence d'un composé organique choisi parmi des éthers, des alcools, des aldéhydes, des amides, et de l'acide formique. L'invention porte également sur un intermédiaire d'héparine dépolymérisée présentant une masse moléculaire comprise entre 1.000 et 5.500, une absorbance à 400 nm inférieure à 0,300, et un rapport S0¿3??-¿/COO?-¿ égal ou supérieur à celui de l'héparine de départ. L'héparine dépolymérisée peut être dissoute dans une solution tampon puis fractionnée par perméation sur gel pour obtenir la masse moléculaire désirée.

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