Process for the preparation of...

C - Chemistry – Metallurgy – 07 – F

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C07F 9/10 (2006.01) C07F 9/09 (2006.01)

Patent

CA 2055503

A process is described for the purification of glycerylphosphorylcholine (GPC) and glycerylphosphorylethanolamine (GPE) from crude deacylated mixtures obtained by deacylation of crude or deoleated soy or egg lecithin or of alcoholic extracts thereof comprising: a) the elution on a cationic resin in acid form equilibrated in anhydrous solvent of the crude solutions from the deacylation step; b) washing of the resin with alcohols or water/alcohols; c) obtaining of the pure mixture nGPC+mGPE by washing the resin with water; d) separation of nGPC+mGPE mixture by elution on basic resins to give pure GPC and GPE.

Procédé de purification de glycérylphosphorylcholine (GPC) et glycérylphosphoryléthanolamine (GPE) à partir de mélanges bruts désacylés obtenus en désacétylant de la lécithine brute ou déshuilée de soja ou d'oeuf ou des extraits alcooliques de cette lécithine. Ce procédé comporte les opérations suivantes: (a) élution d'une résine cationique sous forme acide, équilibrée dans un solvant anhydrique des solutions brutes provenant de l'opération de désacylation; (b) lavage de la résine avec des alcools ou eau/alcools; (c) obtention du mélange pur nGPC+mGPE en lavant la résine avec de l'eau; (d) séparation du mélange nGPC+mGPE par élution sur des résines basiques pour obtenir des GPC et GPE pures.

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