Process for the preparation of a plasminogen activator

Details Process for the preparation of a plasminogen activator Process for the preparation of a plasminogen activator

C - Chemistry, Metallurgy

12

N

195/123

C12N 9/68 (2006.01)

Patent

CA 1206903

ABSTRACT OF THE DISCLOSURE A process for the preparation of a plasminogen activator is described, which comprises bringing normal diploid cells derived from human bodies capable of producing the plasminogen activator into contact with a solution containing an enzymatically-decomposed animal meat peptone, the plasminogen activator having the following properties: a) molecular weight: 63,000 ? 10,000; b) isoelectric point: 7.0 to 8.5; c) affinity to fibrin: present; d) affinity to Concanavalin A: present; e) optimum pH: 7 to 9.5; and f) no reactivity with anti-urokinase specific antibody. The plasminogen activator can be obtained in a high yield.

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