Process for the preparation of a tissue thromboplastin

C - Chemistry – Metallurgy – 12 – Q

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167/104, 195/39.

C12Q 1/56 (2006.01) C07K 14/745 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1195614

ABSTRACT The present invention provides a process for the production or a tissue thromboplastin prepar- ation which is sensitive towards coagulation Factor VII by the production of an acetone dry powder from mammalian tissues and extraction of the powder with salt solution, wherein the extraction is carried out with a salt solution which contains 1 to 20 mMole/litre of calcium ions and optionally a surface-active agent and the extract obtained is, if desired, dried. The present invention also provides a reagent for assessing blood coagulability with a content of thromboplastin and calcium salt, the thromboplastin having been obtained by the above process. If desired, the reagent can also contain a thrombo- plastin preparation which is insensitive towards Factor VII in an amount such that a predetermined Factor VII sensitivity is present.

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