Process for the preparation of freeze-dried micro-organism...

C - Chemistry – Metallurgy – 12 – N

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195/45, 195/82

C12N 1/04 (2006.01) C12N 1/00 (2006.01) C12N 1/02 (2006.01) C12N 1/20 (2006.01)

Patent

CA 1247027

ABREGE DESCRIPTIF Dans un procédé de préparation de concentrés de microorganismes lyophilisés mettant en oeuvre une ultra- filtration avec apport en substrats nutritifs et admission d'agent neutralisant dans le fermenteur, a) on met fin aux apports de substrats en refroidissant: b) on poursuit l'ultra- filtration jusqu'à obtention d'un concentré primaire (40/80 kg poids sec bactérien/m3) et l'on refroidit à 15 - 20°C, c) on lave le concentré primaire avec des sels minéraux et des acides aminés, oligo-éléments et vitamines; d) on concentre par ultrafiltration à 90 - 220 kg poids sec bactérien/m3; e) on refroidit à 5°C et on ajoute un protecteur; f) on congèle et on lyophilise. Application notamment à la prépa- ration de bactéries lactiques.

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