Process for the preparation of human antithrombin iii...

Details Process for the preparation of human antithrombin iii... Process for the preparation of human antithrombin iii...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.3

C12N 15/67 (2006.01) C07K 14/81 (2006.01) C12N 5/10 (2006.01) C12N 15/15 (2006.01) C12N 15/53 (2006.01) C12N 15/85 (2006.01)

Patent

CA 1305932

HOE 86/8 022 ABSTRACT OF THE DISCLOSURE Human antithrombin III (ATIII) which has progressive in- hibitor activity and is stimulated by heparin is advant- geously obtained by expression after cotransfection of mammalian cells whose dihydrofolate reductase (DHFR) ac- tivity is deficient (dhfr-) with a) vectors which contain the ATIII cDNA, and b) vectors which carry the DHFR gene, in particular after gene amplification using methotrexate.

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