Process for the preparation of human insulin or the...

C - Chemistry – Metallurgy – 12 – P

Patent

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195/128.2

C12P 21/04 (2006.01) C07K 14/62 (2006.01)

Patent

CA 1190496

Abstract of the disclosure: The invention provides a process for the preparation of human insulin or the derivatives thereof from pig insulin or the derivatives thereof, which comprises re- acting pig insulin or a derivative thereof at a pH below its isoelectric point with an excess of a threonine ester or one of its derivatives in the presence of trypsin or a trypsin-like enzyme, and optionally splitting off the ester group thereafter.

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