C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/134
C12P 13/04 (2006.01) C12N 9/80 (2006.01) C12P 13/08 (2006.01) C12P 41/00 (2006.01)
Patent
CA 1258244
ABSTRACT The invention relates to a process for the prepar- ation of L-.alpha.-amino acid and D-.alpha.-amino acid amide from DL-.alpha.- amino-acid amide by contacting the DL-.alpha.-amino acid amide in an aqueous solution with an .alpha.-amino acyl amidase containing preparation obtained from a culture of Pseudomonas putida in the presence of traces of bivalent metal ions as activator, characterized in that the aqueous solution also contains a potassium salt selected from the group consisting of potas- sium sulphate and potassium chloride. The invention further relates to a process for the preparation of a L-.alpha.-amino acid and D-.alpha.-amino acid amide starting from the corresponding alde- hyde, potassium cyanide and ammoniumsulphate, subsequent treatment with a ketone and potassiumhydroxide and finally subjecting to enzymatic hydrolysis with a preparation obtained from Pseudomonas putida.
495262
Boesten Wilhelmus H. J.
Kerkhoffs Pieter L.
Fetherstonhaugh & Co.
Stamicarbon B.v. (licensing Subsidiary Of Dsm)
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