C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 17/00 (2006.01) A61K 8/49 (2006.01) A61Q 1/10 (2006.01) A61Q 5/10 (2006.01) C09B 61/00 (2006.01) C09B 67/00 (2006.01) C09B 69/10 (2006.01)
Patent
CA 2128226
The present invention relates to a method for the preparation of a melanic pigment by enzymatic bioconversion, implementing a melanin precursor substrate and plant cells, characterized in that: (a) poppy (Papaver somniferum) plant cells previously cultivated are separated from their culture medium and subcultured in a culture medium with a concentration from 10 to 100 g/l; (b) the cells are ground at least partially, before or at the end of the latency time, (c) the at least partially ground cells are placed in a bioconversion medium in the presence with a melanin precursor substrate selected among indol, indolin, dihydroxyphenylalanin, tyramin and tyrosin; (d) the precipitated melanic pigment is collected. The invention also relates to the utilization of such resulting pigments for the preparation of cosmetic compositions.
2128226 9412653 PCTABS00166 La présente invention concerne un procédé de préparation d'un pigment mélanique par bioconversion enzymatique, mettant en oeuvre un substrat précurseur de mélanine et de cellules végétales, caractérisé en ce que: (a) des cellules végétales de pavot (Papaver somniferum) préalablement cultivées sont séparées de leur milieu de culture et repiquées dans un milieu de culture à une concentration allant de 10 à 100 g/l, (b) on broye les cellules au moins partiellement, avant ou à la fin du temps de latence, (c) on met en présence les cellules au moins partiellement broyées, dans un milieu de bioconversion, avec un substrat précurseur de mélanine choisi parmi l'indole, l'indoline, la dihydroxyphénylalanine, la tyramine et la tyrosine, (d) on récupère le pigment mélanique précipité. Elle concerne également l'utilisation de pigments obtenus pour la préparation de compositions cosmétiques.
Hilaire Pascal
Junino Alex
Martin Richard
L'oreal
Swabey Ogilvy Renault
LandOfFree
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