Process for the preparation of native human glicentin

Details Process for the preparation of native human glicentin Process for the preparation of native human glicentin

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/605 (2006.01) C12N 15/16 (2006.01) C12P 21/06 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2127068

ABSTRACT Disclosed is a process for the preparation of native human glicentin, which comprises producing by recombinant gene technology a fused polypeptide composed of human glicentin having an extra peptide moiety linked to the amino terminus and cleaving the extra peptide moiety in the fused polypeptide, wherein the peptide moiety upon cleavage is designed to constitute even-numbered amino acids and the cleavage of the extra peptide moiety is carried out by hydrolysis with cathepsin C. T7S10 peptide is used as the peptide moiety.

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