C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/00 (2006.01) C07K 14/435 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2420071
The present invention relates to a method for the preparation of a Neutrophil Inhibitory Factor (NIF) comprising the cultivation of mammalian cells expressing NIF in an animal component-free growth medium. The present invention may be employed in large-scale preparation of NIF. The invention also relates to a method for the preparation of recombinant proteins comprising the cultivation of mammalian cells expressing an exogenous recombinant protein in an animal component-free growth medium.
L'invention concerne un procédé de préparation d'un facteur d'inhibition de polynucléaires neutrophiles (NIF) consistant à cultiver des cellules mammifères exprimant un NIF dans un milieu de croissance sans composant animal. Le procédé de cette invention peut être mis en oeuvre dans une préparation de NIF à grande échelle. L'invention concerne également un procédé de préparation de protéines recombinées consistant à cultiver des cellules mammifères exprimant une protéine exogène recombinée dans un milieu de croissance sans composant animal.
Geldart Roderick William
Hawrylik Steven Joseph
Ho Lewis
Koehler Mark Alan
Moyle Matthew
Corvas International Inc.
Mbm Intellectual Property Law Llp
Pfizer Products Inc.
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