Process for the preparation of neutrophil inhibitory factor

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C07K 14/435 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2420071

The present invention relates to a method for the preparation of a Neutrophil Inhibitory Factor (NIF) comprising the cultivation of mammalian cells expressing NIF in an animal component-free growth medium. The present invention may be employed in large-scale preparation of NIF. The invention also relates to a method for the preparation of recombinant proteins comprising the cultivation of mammalian cells expressing an exogenous recombinant protein in an animal component-free growth medium.

L'invention concerne un procédé de préparation d'un facteur d'inhibition de polynucléaires neutrophiles (NIF) consistant à cultiver des cellules mammifères exprimant un NIF dans un milieu de croissance sans composant animal. Le procédé de cette invention peut être mis en oeuvre dans une préparation de NIF à grande échelle. L'invention concerne également un procédé de préparation de protéines recombinées consistant à cultiver des cellules mammifères exprimant une protéine exogène recombinée dans un milieu de croissance sans composant animal.

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