C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) A61K 38/46 (2006.01) C12N 9/22 (2006.01)
Patent
CA 2631400
The present invention relates to a process for the production, by a prokaryotic host cell, of an active, stable transposase of a Mariner mobile genetic element belonging to the mauritiana subfamily. The invention also relates to an active, stable transposase that can be produced using such a process, and to the molecular biology tools, such as the prokaryotic host cells, the expression vectors or the kits, which make it possible to produce an active, stable transposase, or which use it. In addition, the present invention relates to the uses of the active, stable transposases.
La présente invention a pour objet un procédé de production, par une cellule hôte procaryote, d'une transposase active et stable d'un élément génétique mobile mariner appartenant à la sous-famille mauritiana. L'invention concerne également une transposase active et stable susceptible d'être produite à l'aide d'un tel procédé, ainsi que les outils de biologie moléculaire, tels que les cellules hôtes procaryotes, les vecteurs d'expression, les kits, qui permettent de produire une transposase active et stable, ou qui la mettent en oeuvre. En outre, la présente invention vise les utilisations des transposases actives et stables.
Auge-Gouillou Corinne
Bigot Yves
Brillet Benjamin
Germon Stephanie
Centre National de La Recherche Scientifique (cnrs)
Goudreau Gage Dubuc
Universite Francois Rabelais de Tours
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