Process for the production of a heterologous peptide by...

Details Process for the production of a heterologous peptide by... Process for the production of a heterologous peptide by...

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) C07K 14/54 (2006.01) C07K 14/56 (2006.01) C12N 1/21 (2006.01) C12N 9/50 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2379571

The present invention relates to a process for the recombinant production of a desired heterologous polypeptide with a clearly defined homogeneous N-terminus in a bacterial host cell, wherein initially a fusion protein which comprises a peptide with the autoproteolytic activity of an autoprotease N pro of a pestivirus and the heterologous polypeptide is formed in the form of cytoplasmic inclusion bodies in the host cell, and then the inclusion bodies are isolated and treated so that the desired heterologous polypeptide is cleaved autoproteolytically by N pro activity from the fusion protein.

L'invention concerne un procédé de production par recombinaison d'un polypeptide hétérologue désiré, à l'aide d'une terminaison N clairement définie dans une cellule hôte bactérienne. Initialement, une protéine de fusion, qui comprend un peptide possédant l'activité autoprotéolytique d'une autoprotéase N?pro¿ d'un pestivirus et ledit polypeptide hétérologue, est formée sous forme de corps d'inclusion cytoplasmiques dans la cellule hôte, puis les corps d'inclusion sont isolés et traités de sorte que ledit polypeptide hétérologue désiré est clivé de manière autoprotéolytique par une activité N?pro¿ à partir de la protéine de fusion.

Affiliated with

Also associated with

Rating

Process for the production of a heterologous peptide by... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the production of a heterologous peptide by..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the production of a heterologous peptide by... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1759657