Process for the production of a protein

Details Process for the production of a protein Process for the production of a protein

C - Chemistry, Metallurgy

12

N

195/44, 195/1.22

C12N 15/00 (2006.01) C12N 1/20 (2006.01) G01N 33/566 (2006.01)

Patent

CA 1212053

- 1 - Abstract: In a process for the production of a soluble native protein, such as immunoglobulin or methionine-prochymosin, in which an insoluble form of the protein is produced by a host organism transformed with a vector including a gene coding for the protein, the insoluble form of the protein is reversibly denatured in an alkaline aqueous solution at a pH selected to promote dissociation of a group or groups of the protein involved in maintaining the conform- ation of the protein, and the protein is subsequently allowed to renature by reducing the pH of the solution below a pH effective to denature the protein to produce the soluble native form of the protein. The pH of the alkaline aqueous solution is suitably in the range 9.0 to 11.5.

450723

Affiliated with

Also associated with

Rating

Process for the production of a protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the production of a protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the production of a protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1250944