C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/50 (2006.01) A01H 4/00 (2006.01) A61K 38/48 (2006.01) C12N 5/04 (2006.01) C12N 9/08 (2006.01)
Patent
CA 2655067
The present invention relates to the field of biotechnology, and in particular to obtaining secondary metabolites, via induction, such as bromelain from plants of the genus Ananas spp., previously established in vitro. The subject of the invention is the development of an innovative process for extracting bromelain from the natural tissues of the pineapple (Ananas comosus (L) Merr) plant that have been established and induced in vitro with a higher proteolytic activity (200 to 600%) - and in high concentrations - as compared with the protein generated naturally in the plant and currently extracted using conventional methodologies. In this process, use is made of pineapple seedlings established and induced in vitro, which are macerated in a phosphate buffer for the purposes of their extraction. Later, they are subjected to filtering, centrifugation, freezing and lyophilization processes in order thus to obtain a crude bromelain extract characterized by high protein yields and specific activity.
La présente invention concerne le domaine de la biotechnologie et, en particulier, l'obtention de métabolites secondaires par induction, telles que la broméline de plantes du genre Ananasspp., préalablement obtenues in vitro. L'objet de l'invention est de mettre au point un nouveau procédé d'extraction de la broméline des tissus naturels de la plante de l'ananas obtenus et induits in vitro avec une plus grande activité protéolytique (de 200 à 600%) et selon des concentrations élevées, par rapport à la protéine générée de manière naturelle dans la plante et extraite à l'heure actuelle au moyen de procédés classiques. On utilise dans le procédé de l'invention des plantules d'ananas obtenues et induites in vitro, qui sont macérées dans un amortisseur de phosphates pour permettre leur extraction. Lesdites plantules sont ensuites soumises à des processus de filtration, de centrifugation, de congélation et de lyophilisation afin d'obtenir un extrait brut de broméline se caractérisant par des rendements élevés en termes de protéines et d'activité spécifique.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Ortiz Barba Zazil
LandOfFree
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