C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/00 (2006.01) C12M 1/36 (2006.01) C12N 1/20 (2006.01)
Patent
CA 2074466
Process for the production of cell mass and/or fermentation products under sterile conditions, during which the fermentation mixture, at least at times, is recycled and the metabolic products of the cultivated cells, and possibly the cell mass are separated; the process comprises the following steps: - charging the fermentation equipment with a sufficient amount of nutrient medium to start the required cell culture. - sterilizing the equipment as well as adjustment of the required concentration of nutrient medium, - inoculation of the nutrient medium with the starter culture and allowing undisturbed growth of the culture for a defined time, - increasing the concentration of the nutrient medium to the specific nutrient medium concentration of the cell culture by simultaneously increasing the volume of the nutrient medium to the working volume of the fermenter and increasing the concentration of cells, - transition to continuous procedure with exchange of the nutrient medium and separation of the metabolic products as well as complete or partial cell recycling, - termination of the continuous procedure at a required time, harvest of the cell mass under-sterile conditions, and possibly - repetition of some or all of the abovementioned steps in the order stated, as well as a device for the implementation of these processes.
Karl Muller & Co. Kg
Sim & Mcburney
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