Process for the production of insulin by genetically...

Details Process for the production of insulin by genetically... Process for the production of insulin by genetically...

C - Chemistry, Metallurgy

12

P

195/44, 195/128.

C12P 1/00 (2006.01) C07K 14/62 (2006.01)

Patent

CA 1077870

ABSTRACT OF THE DISCLOSURE: A process for the production of insulin using fungal cells. Human insulin producing cells are extracted to obtain the func- tional genome (the genetic material which determines the capacity of the cells to produce insulin) which is used to transform fungal cells, making them capable of producing insulin. The insulin has been extracted and identified by radioimmune asssy and by bioassay. The same process can also be applied to produce animal insulins using specific specie transforming functional insulinogenic genomes. A process for serial secondary culture of insulin producing cells is disclosed. The significance of the invention is related to the great need to produce human insulin in large amounts. Human insulin has distinct biological and clinical advantages when compared ? h commercially available animal insulins now used for the treatment of diabetes mellitus in man.

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