C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/00 (2006.01) C07K 1/113 (2006.01) C07K 14/47 (2006.01) C12N 1/20 (2006.01) C12N 9/72 (2006.01) C12N 15/12 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2304437
A process for the production of a water-soluble, naturally folded eukaryotic polypeptide containing two or several cysteines linked by disulfide bridges, by culturing prokaryotic cells, a) in which the said prokaryotic cells contain an expression vector which encodes the said polypeptide which contains a prokaryotic signal sequence at the N- terminus, b) under conditions under which the polypeptide is secreted into the periplasm or the medium, c) cleaving the signal sequence and isolating the polypeptide from the periplasm or the medium, which is characterized in that the culture is carried out in the presence of arginine or a compound of the general formula I R2-CO-NRR1 (I) in which R and R1 represent hydrogen or a saturated or unsaturated branched or unbranched C1 - C4 alkyl chain and R2 represents hydrogen, NHR1 or a saturated or unsaturated branched or unbranched C1 - C3 alkyl chain, is suitable for the recombinant production of polypeptides in prokaryotes in a high yield.
Ambrosius Dorothee
Rudolph Rainer
Schaffner Jorg
Schwarz Elisabeth
F. Hoffmann-La Roche Ag
Gowling Lafleur Henderson Llp
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