Process for the production of two-chain t-pa

Details Process for the production of two-chain t-pa Process for the production of two-chain t-pa

C - Chemistry, Metallurgy

12

N

195/1.33

C12N 9/68 (2006.01) C12N 9/72 (2006.01)

Patent

CA 2011548

A b s t r a c t In order to produce two-chain t-PA by the culture of eukaryotic cells which are transfected with a vector containing the t-PA gene the culture or culture supernatant is incubated, after reaching the stationary growth phase of the cells, with a re-incubation medium which contains serum or plasma from the horse, ox or foetal calf and a fibrinogen derivative and the t-PA is isolated from the medium.

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