Process for the purification of fc-fusion proteins

Details Process for the purification of fc-fusion proteins Process for the purification of fc-fusion proteins

C - Chemistry, Metallurgy

07

K

C07K 1/36 (2006.01) A61K 38/02 (2006.01)

Patent

CA 2661872

The invention relates to a process for the purification of an Fc-fusion protein having a pI between 6.9 and 9.5 comprising protein A or G affinity chromatography, cation exchange chromatography, anion exchange chromatography and hydroxyapatite chromatography.

La présente invention concerne un procédé de purification d'une protéine FC-hybride présentant un pl compris entre 6,9 et 9,5. Ce procédé peut utiliser une technique de chromatographie d'affinité de protéine A ou G, de chromatographie d'échange de cations, de chromatographie d'échange d'anions et de chromatographie d'adsorption sur gel d'hydroxyapatite.

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