Process for the purification of pharmacologically active...

Details Process for the purification of pharmacologically active... Process for the purification of pharmacologically active...

C - Chemistry, Metallurgy

07

K

C07K 14/56 (2006.01) C07K 1/18 (2006.01) C12N 15/09 (2006.01) C12N 15/20 (2006.01)

Patent

CA 2388716

The object of the invention is a process for the purification of pharmacologically active proteins based on the use of the cationic exchange chromatography on a solid matrix carried out at a more basic pH, i.e. higher, in respect of the pH corresponding to the isoelectric point, pI, of the proteins to be purified, pH at which however said proteins still remain absorbed. Buffer solutions with values of pH and of ionic strength adjusted from time to time to the kind of pharmacologically active protein to be purified are used in order to get such a result. The process is mainly addressed to the purification of the interferon and albumin proteins.

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