C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/39.1
C12N 9/70 (2006.01) C07K 14/315 (2006.01) C12N 9/00 (2006.01)
Patent
CA 2000850
B2619/Abs Abstract A process for the separation of streptokinase from contaminating proteins in a streptokinase-containing mixture, which comprises treating the mixture with a reducing agent to reduce disulphide bridges in the contaminating proteins to free thiol groups, contacting the mixture with a reagent R-X wherein R is a group capable of reacting with a free thiol group and X is a group R1 capable of reacting with a free thiol group or is a thiol-containing matrix, and thereafter separating the resulting chemically modified contaminating proteins from the mixture to provide streptokinase in a form substantially free of contaminating proteins.
Harber Julian S.
Lewis Ceri J.
Mannix Christopher J.
Smith Richard A. G.
Harber Julian S.
Lewis Ceri J.
Mannix Christopher J.
Ridout & Maybee Llp
Roberts Laboratories Inc.
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