Process for the purification of tnf-binding proteins using imac

Details Process for the purification of tnf-binding proteins using imac Process for the purification of tnf-binding proteins using imac

C - Chemistry, Metallurgy

07

K

C07K 14/715 (2006.01) C07K 1/22 (2006.01)

Patent

CA 2505385

A new purification process for Tumor Necrosis Factor-binding proteins is described. In particular this process is characterized by the use as capture step of an Immobilized Metal Affinity Chromatography (IMAC) using copper as metal. This brings advantages in terms of process yields, purity of the final product and applicability to industrial scale.

L'invention concerne un nouveau procédé de purification destiné aux protéines de liaison du facteur de nécrose tumorale. Ce procédé est notamment caractérisé par l'utilisation au stade de capture de la chromatographie d'affinité pour le métal immobilisé (IMAC) utilisant en tant que métal le cuivre. Il présente des avantages en termes de rendement et de pureté du produit final et d'applicabilité à l'échelle industrielle.

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