G - Physics – 01 – N
Patent
G - Physics
01
N
167/45
G01N 33/569 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1278258
ABSTRACT The present invention provides a process standard and kit for the quantification of cell populations or subpopulations, wherein a sample with the cell population to be determined is incubated with labelled antibodies which are specifically directed against characteristic surface antigens of the cell population to be quantified, one or more standards with known, differing particle concentra- tion are incubated with the same labelled antibodies in the same way, the standard solution contains a mixture of particles which, in their sedimentation behaviour, are comparable with the cells to be determined, a first portion of said mixture compris- ing particles carrying a non-specific antibody and a second portion comprising particles carrying mole- cules directed specifically against the labelled antibodies or a part thereof, the cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies, the amount of the labelling is measured not only on the cells but also on the particles and by comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells
521967
Albert Winfried
Endl Josef
Schetters Hartmut
Albert Winfried
Boehringer Mannheim Gmbh
Endl Josef
Schetters Hartmut
Swabey Ogilvy Renault
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