C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.1, 150/3.
C12Q 1/48 (2006.01)
Patent
CA 1129316
Abstract In a process for the quantative enzymatic deter- mination of adenosine diphosphate (ADP) in aqueous solution by phosphorylation of the ADP with a kinase in the presence of a phosphorylation substrate therefor, with the formation of ATP and a dephoaphorylated sub- strate, such as galactose, formate, pyruvate or nucleoside diphosphate, the dephosphorylated substrate is reacted, optionally with the insertion of a further adjutant reaction, such as a decarboxylation, trans- amination or hexosidation, with NAD(P) in the presence of a dehgdrogenage and the NAD(P)H formed either measured directly or reacted with a tetrazolium salt in the presence of an electron carrier, with the formation of a formazane, which latter is then measured. A reagent suitable for carrying out this process contains a kinase, a phosphorylated substrate therefor different from ADP, a specific dehydrogenase for the dephosphorylated form of the substrate or for a trans- aminated, decarboxylated or hexosidised reaction product thereof, the co-enzyme of the dehydrogenase and a buffer, as well as optionally a tetrazolium salt, an electron carrier suitable therefor and optionally a surface-active agent.
331506
Beutler Hans-Otto
Deneke Ulfert
Michal Gerhard
Boehringer Mannheim G.m.b.h.
Sherman
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