Process for the removal of selectable marker gene sequences

C - Chemistry – Metallurgy – 12 – N

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C12N 15/90 (2006.01)

Patent

CA 2572003

The invention relates to a process for the removal of selectable marker gene sequences, in particular antibiotic gene sequences, from nucleic acid molecules, wherein the process makes use of dif-like site-specific recombinase recognition sites. The invention further relates to the application of this process in the unlabelled integration and deletion of chromosomal genes and in controlling gene expression.

Procédé d'élimination de séquences de gènes marqueurs sélectionnables, plus particulièrement de séquences de gènes antibiotiques, provenant de molécules d'acides nucléiques. Le procédé fait intervenir des sites de recombinase spécifique aux sites de type dif. Application de ce procédé dans l'intégration et la suppression non marquées de gènes chromosomiques et dans le contrôle de l'expression génique.

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