Process for the separation of l-leucine and l- isoleucine

Details Process for the separation of l-leucine and l- isoleucine Process for the separation of l-leucine and l- isoleucine

C - Chemistry, Metallurgy

12

P

195/64, 195/134.

C12P 13/06 (2006.01) C07C 227/00 (2006.01) C07C 227/28 (2006.01) C07C 227/40 (2006.01) C07C 229/00 (2006.01) C07C 229/04 (2006.01)

Patent

CA 1263094

0455p de Roos Case 1 PROCESS FOR THE SEPARATION OF L-LEUCINE AND L-ISOLEUCINE Abstract of the Invention The invention relates to a process for the separation of L-leucine and L-isoleucine. The process is carried out,by sub- jecting a mixture of leucine ester, and isoleucine ester, (or a mixture of leucine ester, isoleucine ester and valine ester) to an esterase catalyzed hydrolysis resulting in a selective hy- drolysis of the leucine ester. The pure L-leucine can be iso- lated from the reaction mixture by first removing the non- hydrolyzed esters by extraction with an organic solvent and then crystallizing the L-leucine at its isoelectric point. The recovered L-iso-leucine ester is purified by distillation (if necessary), and hydrolyzed to yield pure crystalline L-isoleucine.

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