Process for transforming a human insulin precursor to human...

C - Chemistry – Metallurgy – 12 – P

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195/128.2

C12P 21/06 (2006.01) C07K 14/62 (2006.01)

Patent

CA 1339955

This specification describes a process for converting a human insulin precursor to human insulin, which comprises treating such human insulin precursor with trypsin and carboxypeptidase B in an aqueous medium containing per mole of human insulin precursor from about 0.1 to about 10 moles of one or more metal ions of those metals having Atomic Numbers 21 to 34, 39 to 52, 57 to 84, and 89 to 92.

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