Process for transforming gramineae and the products thereof

Details Process for transforming gramineae and the products thereof Process for transforming gramineae and the products thereof

C - Chemistry, Metallurgy

12

N

47/4, 195/45, 19

C12N 15/90 (2006.01) A01H 1/00 (2006.01) A01H 1/02 (2006.01) A01H 5/00 (2006.01) A01H 5/08 (2006.01) A01H 5/10 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/52 (2006.01) C12N 15/82 (2006.01) C12N 15/84 (2006.01) C12N 15/87 (2006.01)

Patent

CA 1341455

A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and in- oculating the wound with vir + Agrobacterium tumefaciens. Also, this same method wherein the vir + A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir + A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir + A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir + Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir + A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir + Agrobacterium tumefaciens and transformed Gramineae derived from seedlings in- fected with vir + A. tumefaciens containing a vector comprising genetically-engineered T-DNA.

540844

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