Process for treating eucaryotic cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/87 (2006.01) A61K 31/70 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2183647

In a method of treating eukaryotic cells, for applications in which the toxicity of the lipopolysaccharide has a detrimental effect, substances which bind lipopolysaccharide and neutralise its toxicity are used during transfection or in order to purify the foreign material. The process is of particular benefit when foreign material is introduced into the cells, particularly in gene transfer methods. Compositions containing a lipopolysaccharide-binding substance may be used in cell culture applications as a medium and in therapeutic applications as a drug.

Dans un procédé de traitement de cellules eucaryotes, dans des applications où la toxicité du lipopolysaccharide est néfaste, des substances fixant le lipopolysaccharide et neutralisant sa toxicité sont utilisées pendant la transfection ou pour purifier le matériau étranger. Ce procédé est avant tout avantageux lorsqu'un matériau étranger est introduit dans les cellules, notamment dans les procédés de transfert de gènes. Des compositions contenant une substance fixant le lipopolysaccharide s'utilisent comme milieu dans les applications de culture cellulaire, et comme médicaments dans les applications thérapeutiques.

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