C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) A01H 5/00 (2006.01) C07K 14/315 (2006.01) C07K 19/00 (2006.01) C12N 5/04 (2006.01) C12N 15/09 (2006.01) C12N 15/29 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01) C12N 15/64 (2006.01) C12N 15/67 (2006.01) C12N 15/82 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2376392
A process of expressing a recombinant protein in a plant and of isolating the recombinant protein from the plant, the process is effected by (a) providing a plant, a plant derived tissue or cultured plant cells expressing a fusion protein including the recombinant protein and a cellulose binding peptide being fused thereto, the fusion protein being compartmentalized within cells of the plant, plant derived tissue or cultured plant cells, so as to be sequestered from cell walls of the cells of the plant, plant derived tissue or cultured plant cells; (b) homogenizing the plant, plant derived tissue or cultured plant cells, so as to bring into contact the fusion protein with a cellulosic matter of the plant, plant derived tissue or cultured plant cells, to thereby effect affinity binding of the fusion protein via the cellulose binding peptide to the cellulosic matter, thereby obtaining a fusion protein cellulosic matter complex; and (c) isolating the fusion protein cellulosic matter complex.
L'invention concerne un procédé pour exprimer une protéine recombinante dans une plante et pour isoler la protéine recombinante de cette plante, le procédé consistant en ce qui suit: (a) fournir une plante, un tissu dérivé de la plante ou des cellules de culture provenant de la plante exprimant une protéine de fusion qui comprennent la protéine recombinante et un peptide de cellulose fusionnée à celle-ci, la protéine de fusion étant cloisonnée à l'intérieur des cellules de la plante, du tissu dérivé de la plante ou des cellules de culture provenant de la plante; (b) homogénéiser la plante, le tissu dérivé de la plante ou les cellules de culture provenant de la plante de manière à mettre la protéine de fusion en contact avec la matière cellulosique de la plante, lu tissu dérivé de la plante ou les cellules de culture provenant de la plante, et ce pour effectuer la liaison par affinité de la protéine de fusion, à travers le peptide liant la cellulose, avec la matière cellulosique pour obtenir un complexe de matière cellulosique et de protéine de fusion; (c) et, enfin, isoler le complexe de matière cellulosique et de protéine de fusion.
Shani Ziv
Shoseyov Oded
Cbd Technologies Ltd.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Yissum Research Development Company Of The Hebrew University In
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