Process of manufacturing enzyme preparation rich in lipase

Details Process of manufacturing enzyme preparation rich in lipase Process of manufacturing enzyme preparation rich in lipase

C - Chemistry, Metallurgy

12

P

195/123, 195/74

C12P 21/00 (2006.01) C12N 9/20 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1047422

ABSTRACT OF THE DISCLOSURE Enzyme preparation rich in lipase is obtained by treating comminuted pancreas tissue with a mixture of about 9 parts by volume of chloroform and one part butanol. The partly degreased tissue is then left standing at 0-4°C for 24 to 96 hours. Subsequently, it is degreased through treat- ment with acetone and is then dehydrated, which is followed by an extraction with a 5% aqueous ethanol solution. The extract is then mixed with acetone, the obtained precipitate is isolated and dried.

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