Process of producing a plastid-targeted protein in plant cells

Details Process of producing a plastid-targeted protein in plant cells Process of producing a plastid-targeted protein in plant cells

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01)

Patent

CA 2526911

A process of producing a protein of interest in cells of a predetermined plant, comprising: introducing into said cells a vector encoding a fusion protein comprising from the N-terminus to the C-terminus (i) a transit peptide for targeting said fusion protein into plastids and, contiguous thereto, (ii) said protein of interest, wherein the C-terminal three amino acids X-3X-2X-1 of said transit peptide and the N-terminal amino acid Z of said protein of interest form a cleavage site X-3X-2X-1-Z for cleaving said fusion protein between X-1 and Z for releasing said protein of interest in plastids, whereby for a predetermined amino acid Z of said protein of interest an amino acid sequence X-3X-2X-1 is selected from the set of amino acid sequences X-3X-2X-1 naturally occurring contiguous to Z in plastid-targeted fusion proteins in plants, thereby forming said cleavage site.

L'invention concerne un procédé de production d'une intéressante dans des cellules d'une plante prédéterminée, comprenant : l'introduction, dans lesdites cellules, d'un vecteur codant une protéine de fusion comprenant, de la terminaison N à la terminaison C : (i) un peptide de transit pour le ciblage de ladite protéine de fusion dans des plastides et, contiguë à celui-ci, (ii) ladite protéine intéressante, procédé caractérisé en ce que les trois aminoacides de la terminaison C, X?-3¿ X?-2¿ X?-1¿, dudit peptide de transit, et l'aminoacide Z de la terminaison N, de ladite protéine intéressante, forment un site de clivage X?-3¿ X?-2¿X?-1¿ Z pour le clivage de ladite protéine de fusion entre X?-1¿ et Z en vue de libérer ladite protéine intéressante dans des plastides, et en ce que, pour un aminoacide prédéterminé Z de ladite protéine intéressante, une séquence X?-3¿X?-2¿X?-1¿ est sélectionnée à partir de la série de séquences aminoacides X?-3¿X?-2¿X?-1¿ se présentant à l'état naturel, contiguës à Z, dans des protéines de fusion ciblées dans des plastides, dans des plantes, de manière à former ledit site de clivage.

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