Process to increase the production of clavam antibiotics

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12P 17/18 (2006.01)

Patent

CA 2221518

A process for increasing the amount of clavam produced by an organism having both a clavam pathway or a portion thereof and a cephalosporin pathway or a portion thereof by interfering with the conversion of L-lysine to L- .alpha.- aminoadipic acid in the cephalosporin pathway. Plasmids containing a defective LAT (lysine amino transferase) gene and organisms containing such plasmids are also provided.

La présente invention concerne un procédé permettant d'augmenter la quantité de clavam produite par un organisme comportant à la fois une voie de clavam ou une partie de celle-ci ainsi qu'une voie de céphalosporine ou une partie de celle-ci, en interférant avec la conversion de L-lysine en acide L- alpha -aminoadipique dans la voie de la céphalosporine. L'invention concerne également des plasmides contenant un gène défectueux de LAT (lysine amino transferase) ainsi que les organismes contenant de tels plasmides.

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