Processes and vectors for plastid transformation

Details Processes and vectors for plastid transformation Processes and vectors for plastid transformation

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2433830

This invention discloses a novel process and vector therefore for producing a multicellular plant or plant cells having stably transformed plastids. This process comprises transforming plastids or plant cells via homologous recombination with a DNA molecule enabling DNA modification, whereby said DNA molecule comprises a fragment of a gene requiring for expression a sequence element of the host plastid. The invention also includes the use of novel selection inhibitors for plastic transformation and the possibility of performing multiple rounds of transformation without accumulation of resistance genes.

Cette invention concerne un procédé et un vecteur permettant de produire un végétal multicellulaire ou des cellules végétales comportant des plastes transformés de manière stable. Ce procédé consiste à transformer des plastes ou des cellules végétales au moyen d'une recombinaison homologue effectuée avec une molécule d'ADN permettant une modification d'ADN, ladite molécule d'ADN comprenant un fragment d'un gène qui, pour son expression, nécessite un élément de séquence du plaste hôte. La présente invention concerne également l'utilisation d'une nouvelle sélection d'inhibiteurs de la transformation de plastes, ainsi que la possibilité d'effectuer de multiples cycles de transformation sans que des gènes de résistance soient accumulés.

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