Processes and vectors for plastid transformation of higher...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2433882

A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.

L'invention concerne un procédé de production de végétaux multicellulaires, d'organes végétaux ou de tissus végétaux transformés au niveau de leur plastome grâce aux étapes suivantes consistant : (a) à modifier ou interrompre la fonction d'un gène dans un génome plastidial pour produire un phénotype sélectionnable ou reconnaissable ; (b) à isoler ou à choisir des végétaux ou des cellules contenant des plastes qui expriment ledit phénotype ; (c) à transformer ledit génome plastidial dudit végétal, organe végétal ou tissu végétal isolé ou choisi au moyen d'au moins un vecteur de transformation présentant une séquence de restauration pouvant restaurer ladite fonction ; et (d) à isoler ou à choisir ledit végétal, organe végétal ou tissu végétal transformé contenant des plastes qui expriment ladite fonction restaurée.

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