Processes and vectors for producing transgenic plants

Details Processes and vectors for producing transgenic plants Processes and vectors for producing transgenic plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2450665

A process for producing transgenic plants or plant cells capable of expressing a coding sequence of interest under transcriptional and translational control of host nuclear transcriptional and translational elements is described by introducing into the nuclear genome of host plants or plant cells a vector comprising said coding sequence of interest which is devoid of (a) an upstream element of initiation of transcription functional in the host plants or plant cells and operably linked to said coding sequence of interest and required for its transcription; (b) an upstream element of initiation of translation functional in the host plants or plant cells and operably linked to said coding sequence of interest; and subsequently selecting plant cells or plants expressing said coding sequence of interest.

L'invention concerne un procédé de production de plantes transgéniques ou de cellules de plantes pouvant exprimer une séquence de codage intéressante sous contrôle transcriptionnel et translationnel des éléments de transcription et translation nucléaires hôtes. Ce procédé consiste à introduire, dans le génome nucléaire des plantes hôtes ou des cellules des plantes, un vecteur comprenant ladite séquence de codage intéressante. Ce vecteur est dépourvu (a) d'un élément amont d'initiation de la transcription fonctionnelle dans les plantes hôtes ou les cellules de plantes et lié de manière opérationnelle à ladite séquence de codage et requis pour sa transcription ; (b) d'un élément amont d'initiation de la transcription fonctionnelle dans les plantes hôtes ou les cellules de plantes et lié de manière opérationnelle à ladite séquence de codage . Ce procédé consiste ensuite à sélectionner des cellules de plantes ou des plantes exprimant ladite séquence de codage.

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