Processes for inhibiting and for inducing flower formation...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/60 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/88 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2184741

Processes for inhibiting flower formation and processes for inducing flower formation in plants, and processes for improving the storage capability of storage organs of useful plants and processes for reducing the sprouting of tubers in tuberous plants are described. Also described are DNA sequences which modify the activity of the citrate synthase of the plant upon integration into a plant genome, plasmids which contain these DNA sequences and transgenic plants in which modifications in the activity of the citrate synthase are brought about by introducing the DNA sequences. The described DNA sequences are sequences from Solanum tuberosum, Nicotiana tabacum and Beta vulgaris which code for the enzyme citrate synthase. The invention also describes transgenic potato plants in which an inhibition of flower formation, a reduction in the storage losses of the tubers and a reduction in the sprouting of the tubers comes about because of an inhibition of the citrate synthase activity, and transgenic potato plants in which a premature induction of flower production comes about because of the over-expression of a citrate synthase.

L'invention se rapporte à des procédés d'inhibition de la floraison et à des procédés de déclenchement de la floraison des plantes, ainsi que des procédés d'amélioration de la capacité de stockage d'organes de plantes utiles, et à des procédés de la germination des tubercules dans des plantes tubérifères. L'invention se rapporte également à des séquences d'ADN qui modifient l'acitivité de la synthase du citrate de la plante lors de l'intégration dans un génome végétal, des plasmides qui contiennent ces séquences d'ADN, et des plantes transgéniques dans lesquelles les modifications de l'activité de la synthase du citrate sont apportées par introduction des séquences d'ADN. Les séquences d'ADN décrites sont des séquences issues de Solanum tuberosom, Nicotiana tabacum et Beta vulgaris qui codent pour la synthase du citrate. L'invention se rapporte également à des plants de pommes de terre transgéniques dans lesquels se produisent une inhibition de la floraison, une réduction des pertes de stockage des tubercules ainsi qu'une réduction de la germination des tubercules du fait d'une inhibition de l'activité de la synthase du citrate, et à des plants de pommes de terre transgéniques dans lesquels se produit un déclenchement prématuré de la floraison du fait de la surexpression d'une synthase de citrate.

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